Premium
PRODUCTS OF CHLOROPHYLL PHOTODEGRADATION–1. DETECTION and SEPARATION
Author(s) -
Llewellyn Carole A.,
Mantoura R. Fauzi C.,
Brereton Richard G.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01823.x
Subject(s) - photodegradation , chemistry , chromatography , aqueous solution , chlorophyll , chlorophyll a , fraction (chemistry) , suspension (topology) , size exclusion chromatography , pigment , adsorption , photochemistry , organic chemistry , biochemistry , catalysis , photocatalysis , mathematics , homotopy , pure mathematics , enzyme
— The photodegradative fate of 14 C‐radiolabelled phaeophytin a adsorbed on hydrophobic particles in an aqueous suspension is followed. An approximate mass balance is made for the hydrophilic, hydrophobic and CO, fractions. In addition, components of the colourless hydrophilic fraction produced from photodegrading chlorophyll a, phaeophytin a, phaeophorbide a and chlorophyll b are detected and separated using reverse‐phase ion‐pair and ion‐exclusion HPLC with UV detection. The increase in the hydrophilic molecules and the decrease in chlorophyll a are measured as the photodegradation proceeds.