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ULTRAVIOLET RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN
Author(s) -
Netto Madeline M.,
Fodor Stephen P. A.,
Mathies Richard A.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01806.x
Subject(s) - bacteriorhodopsin , raman spectroscopy , resonance (particle physics) , resonance raman spectroscopy , chemistry , spectroscopy , ultraviolet , analytical chemistry (journal) , photochemistry , nuclear magnetic resonance , materials science , optics , atomic physics , optoelectronics , organic chemistry , physics , biochemistry , quantum mechanics , membrane
— Ultraviolet resonance Raman spectra of bacteriorhodopsin have been obtained using 229 nm excitation from a hydrogen‐shifted neodymium yttrium aluminum garnet (Nd: YAG) laser. High signal‐to‐noise spectra are observed exhibiting vibrational bands at 762, 877, 1011, 1175, 1356, 1552 and 1617 cm −1 which are assigned to scattering from tryptophan and tyrosine side chains. This demonstrates the feasibility of using UV resonance Raman spectroscopy to monitor aromatic amino acid structural changes during the bacteriorhodopsin photocycle.