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ACTION SPECTRA FOR THE GENERATION OF SINGLET OXYGEN FROM MITOCHONDRIAL MEMBRANES FROM SOYBEAN (Glycine max) HYPOCOTYLS
Author(s) -
Jung Jin,
Kim HeeJung,
Cho Moonjae
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01800.x
Subject(s) - singlet oxygen , glycine , flavoprotein , action spectrum , chemistry , absorption (acoustics) , photochemistry , absorption spectroscopy , oxygen , biophysics , biochemistry , materials science , biology , amino acid , physics , organic chemistry , optics , composite material , enzyme
— The action spectrum for the generation of singlet oxygen ( 1 O 2 ) from mitochondrial membranes under aerobic conditions was measured at wavelengths between 360 and 600 nm, using sub‐mitochondrial particles (SMP) prepared from soybean hypocotyls. The spectrum, showing a peak at about 420 nm, remarkably resembles the absorption spectra of the Fe‐S centers of nonheme iron proteins. Disruption of the Fe‐S centers by treating SMP with mersalyl acid resulted in a substantial decrease in the efficiency of 1 O 2 generation, leaving an action spectrum whose pattern is significantly similar to the absorption spectrum of flavins, at least in the region of near UV and blue light wavelengths. Estimating the contribution of the Fe‐S centers to the generation of 1 O 2 from SMP, we suggest that the Fe‐S centers act as very important endogenous photosensitizers in plant cells, in so far as the type II mechanism is concerned. Possible involvement of mitochondrial flavoproteins in the generation of 1 O 2 is also discussed.