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THE USE OF STOKES RAMAN SCATTERING IN TIME CORRELATED SINGLE PHOTON COUNTING: APPLICATION TO THE FLUORESCENCE LIFETIME OF TYROSINATE *
Author(s) -
Willis Kevin J.,
Szabot Arthur G.,
Krajcarski Donald T.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01725.x
Subject(s) - rayleigh scattering , raman scattering , photon counting , fluorescence , excitation , scattering , stokes shift , raman spectroscopy , chemistry , laser , photon , molecular physics , optics , analytical chemistry (journal) , atomic physics , physics , quantum mechanics , chromatography
— The decay kinetics of the tyrosinate form of tyrosine in aqueous solution, pH 11, were investigated by time correlated single photon counting using laser excitation. The lifetime of the single exponential decay was 30.5 ± 0.2 ps. Stokes Raman scattering was used to determine the instrument response function and the advantages of this approach over the conventional Rayleigh scattering method are discussed.