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THE EFFECTS OF LOW DENSITY LIPOPROTEINS ON UPTAKE OF PHOTOFRIN II
Author(s) -
Korbelik Mladen,
Hung Jaclyn,
Lam Stephen,
Palcic Branko
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01702.x
Subject(s) - internalization , chemistry , fluorescence , extraction (chemistry) , biophysics , biochemistry , chromatography , biology , cell , physics , quantum mechanics
— The cellular uptake of Photofrin II (PII) was studied using fluorescence imaging and chemical extraction. The influence of serum and low density lipoproteins (LDL) was examined under a variety of experimental conditions employing cultured human cells of different origins as well as a subcutaneously SMT‐F tumor implanted in mice. Results showed that serum inhibited PII uptake. In general, LDL also inhibits PII uptake with the exception of an initial increase in the first10–30 min when the cellular concentration of PII was measured by fluorescence imaging instead of chemical extraction. Our results suggest a possible de‐aggregation process occurring upon internalization or binding of PII to LDL.