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FLUORESCENCE QUENCHING OF INDOLIC COMPOUNDS IN REVERSE MICELLES OF AOT
Author(s) -
Lissi E. A.,
Encinas M. V.,
Bertolotti S. G.,
Cosa J. J.,
Previtali C. M.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01683.x
Subject(s) - chemistry , micelle , indole test , tryptophan , quenching (fluorescence) , fluorescence , heptane , photochemistry , partition coefficient , chromatography , organic chemistry , aqueous solution , biochemistry , physics , amino acid , quantum mechanics
Abstract— The fluorescence properties of indole, 1,2‐dimethylindole and tryptophan have been determined in sodium bis(2‐ethylhexyl)sulfosuccinate inverse micelles in heptane. Fluorescence quenching studies were done using CCl 4 and acrylamide as quenchers localized in the organic and micellar pseudophase respectively. The Stern‐Volmer plots show departure from linearity and are dependent on the emission wavelength. The results were interpreted using a three pseudophases model. 1,2‐Dimethylindole is predominantly solubilized in the n ‐heptane; however a small interaction with the micelles is apparent at high detergent concentration. Indole is distributed between the organic and the interfacial microphase with a partition constant of 34 ± 3, independent of the water content. Tryptophan is entirely localized at the interface. However the quenching experiments show that tryptophan molecules are not uniformly distributed in the interfacial pseudophase.