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LONG WAVE ULTRAVIOLET RADIATION STIMULATES ARACHIDONIC ACID RELEASE AND CYCLOOXYGENASE ACTIVITY IN MAMMALIAN CELLS IN CULTURE
Author(s) -
HANSON DEBRA L.,
DELEO VINCENT A.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb09190.x
Subject(s) - arachidonic acid , phospholipase a2 , biochemistry , chemistry , phosphatidic acid , cyclooxygenase , choline , phospholipase , phospholipase a , liberation , enzyme , membrane , phospholipid , in vitro
— Human fibroblasts and mouse C3H 10T1/2 cells in culture were prelabeled with [ 3 H]arachidonic acid and exposed to UVA radiation. Cells released labeled arachidonate metabolites into medium in a dose‐dependent fashion (5–20 J ctrr 2 ). The time course of release appeared biphasic with peak responses occurring immediately and at 2 h post irradiation. Release of radiolabel was oxygen and calcium ion dependent and was inhibited by the addition of phenylglyoxal. indomethacin, and dibucaine to the medium. High performance liquid chromatographic examination of medium extracts revealed UVA stimulation of cyclooxygenase metabolism of [ 3 Hjarachidonic acid and specifically, prostaglandin E 2 production by cells in culture. Furthermore, UVA stimulated a dose‐dependent release of membrane incorporated pH]choline from cells in culture. Paper chromatographic analysis of the medium provided evidence that choline release from the membrane was predominantly accompanied by release of phosphorylcholine with some glycerophosphorylcholine suggesting indirectly that the major pathway for UVA‐stimulated arachidonic acid release was via phospholipase C and diacylglycerol lipase enzyme systems.