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MORE EFFICIENT EXCISION REPAIR OF PYRIMIDINE DIMERS IN THE SPECIFIC DNA SEQUENCE THAN IN THE GENOME OVERALL IN GOLDFISH CELLS
Author(s) -
KOMURA JUNICHIRO,
MITANI HIROSHI,
SHIMA AKIHIRO
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb09189.x
Subject(s) - pyrimidine dimer , agarose gel electrophoresis , micrococcus luteus , dna , microbiology and biotechnology , genome , gene , biology , nucleotide excision repair , sequence (biology) , transfection , gel electrophoresis , agarose , endonuclease , chemistry , genetics , dna damage , escherichia coli
— Excision repair of pyrimidine dimers induced by 254 nm UV was examined in the genome overall and in a specific sequence containing a transfected gene for hygromycin B resistance, in RBCF‐1 cells derived from a goldfish, by the use of UV endonuclease of Micrococcus luteus and alkaline agarose gel electrophoresis. More than 40% of dimers were removed from the specific sequence, while about 20% were removed from the genome overall, within 24 h after exposure to UV (2.5–7.5 J/m 2 ).