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PRODUCTION OF MALONALDEHYDE FROM SQUALENE, A MAJOR SKIN SURFACE LIPID, DURING UV‐IRRADIATION
Author(s) -
DENNIS K. JASON,
SHIBAMOTO TAKAYUKI
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb08445.x
Subject(s) - squalene , chemistry , irradiation , lipid peroxidation , thiobarbituric acid , ultraviolet irradiation , ultraviolet , nuclear chemistry , chromatography , biochemistry , antioxidant , physics , nuclear physics , quantum mechanics
Malonaldehyde (MA), a product of lipid peroxidation, is known to have a number of toxicological effects. Among skin surface components, squalene has been suggested as a significant source of MA following UV exposure. Irradiation of squalene coated on the inside surface of glass tubes served as a simple model to examine skin surface photochemistry. Malonaldehyde produced during UV‐irradiation was reacted with methyl hydrazine to form N‐methylpyrazole, which was then measured by gas chromatographic analysis. Ultraviolet A‐irradiation produced low levels of MA from squalene, with a maximum of 1.0 nmol MA/µmol squalene near 6 h. Malonaldehyde production varied with the energy of irradiation, with levels of 176 nmol/mg squalene and 9 nmol/mg squalene produced after 3 h of irradiation with UVB and UVA, respectively. The thiobarbituric acid (TBA) assay for lipid peroxidation showed large overestimations of MA levels under all irradiation conditions tested. The TBA assay predicted the highest levels of MA with UVC but we did not detect any MA with our assay.