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UV LASER INDUCED RNA‐PROTEIN CROSSLINKS AND RNA CHAIN BREAKS IN TOBACCO MOSAIC VIRUS RNA in situ
Author(s) -
DOBROV E. N.,
ARBIEVA Z. KH.,
TIMOFEEVA E. K.,
ESENALIEV R. O.,
ORAEVSKY A. A.,
NIKOGOSYAN D. N.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb08429.x
Subject(s) - rna , irradiation , picosecond , nanosecond , tobacco mosaic virus , chemistry , photochemistry , laser , in situ , quantum yield , biophysics , virus , biology , virology , biochemistry , optics , physics , organic chemistry , nuclear physics , fluorescence , gene
The efficiency of RNA‐protein crosslink and RNA chain break formation under nanosecond or picosecond UV‐laser pulse irradiation of tobacco mosaic virus was determined. It was found that on high‐intensity UV‐laser irradiation the quantum yields of both reactions increase considerably as compared to the usual (low‐intensity) UV‐irradiation. The RNA‐protein crosslink quantum yield was found to be 1.8 × 10 ‐5 and 1.2 × 10 ‐4 and that of RNA chain breaks 1.7 × 10 ‐4 and 8.9 × 10 ‐4 for nanosecond and picosecond irradiation, respectively.