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PHOTO‐CIDNP OF THE AMINO ACIDS
Author(s) -
STOB S.,
KAPTEIN R.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb08425.x
Subject(s) - cidnp , tryptophan , chemistry , histidine , tyrosine , photochemistry , amino acid , methionine , aromatic amino acids , stereochemistry , biochemistry , radical
A photochemically induced dynamic nuclear polarisation (photo‐CIDNP) study is presented of the amino acids that are polarisable with a flavin dye. These include derivatives of tryptophan, tyrosine, histidine, methylated lysines and methionine. The influence of pH, concentration and chemical modification on the magnitude of the CIDNP effect has been studied to obtain mechanistic information about the radical pair formation. The pH and concentration dependence of tyrosine and tryptophan polarisation could be accounted for quantitatively. The CIDNP evidence indicates that hydrogen‐atom abstraction is important in generating radical pairs in the case of histidine and tyrosine, while electron transfer prevails in the case of tryptophan, the methylated lysines and methionine.