Premium
FLOW CYTOMETRIC ANALYSIS OF INTRACELLULAR HEMATOPORPHYRIN DERIVATIVE IN HUMAN TUMOR CELLS AND MULTICELLULAR SPHEROIDS
Author(s) -
West Catharine M. L.,
Moore James V.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04324.x
Subject(s) - hematoporphyrin , spheroid , intracellular , multicellular organism , chemistry , microbiology and biotechnology , flow cytometry , tumor cells , derivative (finance) , biophysics , biology , cell , in vitro , cancer research , biochemistry , photodynamic therapy , organic chemistry , financial economics , economics
— Flow cytometry (FCM) has been used to investigate the intracellular fluorescence of hematoporphyrin derivative (HPD) in monolayer and spheroid cultures of WiDr cells. For exponentially‐growing monolayer cultures mean cellular fluorescence was directly proportion to the external HPD levels in the range 5–100 µg ml −1 (r = 0.99). Heterogeneity of cellular fluorescence was quantified by determining the ratio of the fluorescence value below which were observed values for 98% of the cell population compared to the fluorescence value for 2%. In exponentially‐growing cultures, decreasing levels of HPD in the medium led to an increase in the 98:2% ratio, i.e. an increase in heterogeneity of intracellular drug levels. The growth of cells as multicellular spheroids confers a spheroid‐size‐dependent resistance to photodynamic treatment. With increasing spheroid size (100, 250, 500, 750 and 1000 µm diam.) there was a decrease in mean intracellular HPD levels and a large linear increase in the 98:2% ratio (r = 0.94).