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USE OF CHARGE COUPLED DEVICE CAMERA FOR IMAGING OF INTRACELLULAR PHTHALOCYANINES
Author(s) -
Chan W.S.,
MacRobert A. J.,
Phillips D.,
Hart I. R.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04317.x
Subject(s) - fluorescence , intracellular , cytochemistry , phthalocyanine , immunofluorescence , chemistry , charge coupled device , biophysics , macrophage , optoelectronics , materials science , photochemistry , optics , biochemistry , nanotechnology , biology , physics , in vitro , immunology , antibody , enzyme
— A highly sensitive charge‐coupled device (CCD) camera, linked to a computerized image processor, has been used to obtain fluorescence images of chloro‐aluminum sulfonated phthalocyanine (ClAlSPc), and its mono‐ to tetra‐sulfonated constituents, in cells recovered from the peritoneal cavity of dye‐treated mice. Immunofluorescence and cytochemistry showed that cells which preferentially took up ClAlSPc and its sulfonated species were predominantly of the monocyte‐macrophage series. While this technique may be of use in determining the nature of those cells capable of taking up light sensitive dyes and, perhaps, the intracellular location of such agents, the possibility that photoinactiv‐ation by dye aggregation could reduce the sensitivity of this procedure is discussed.