Premium
REACTIVITY OF CHLOROPHYLL a/b‐PROTEINS AND MICELLAR TRITON X‐100 COMPLEXES OF CHLOROPHYLLS a OR b WITH BOROHYDRIDE
Author(s) -
Scheer Hugo,
Porra Robert J.,
Anderson Jan M.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04177.x
Subject(s) - reactivity (psychology) , chemistry , chlorophyll , chlorophyll a , p700 , chlorophyll b , monomer , photochemistry , borohydride , photosynthesis , organic chemistry , photosystem i , photosystem ii , biochemistry , polymer , catalysis , medicine , alternative medicine , pathology
— The reaction of several plant chlorophyll‐protein complexes with NaBH 4 has been studied by absorption spectroscopy. In all the complexes studied, chlorophyll b is more reactive than Chi a, due to preferential reaction of its formyl substituent at C‐7. The complexes also show large variations in reactivity towards NaBH 4 and the order of reactivity is: LHCI > PSII complex > LHCII > PSI > P700 (investigated as a component of PSI). Differential pools of the same type of chlorophyll have been observed in several complexes. Parallel work was undertaken on the reactivity of micellar complexes of chlorophyll a and of chlorophyll b with NaBH 4 to study the effect of aggregation state on this reactivity. In these complexes, both chlorophyll a and b show large variations in reactivity in the order monomer > oligomer > polymer with chlorophyll b generally being more reactive than chlorophyll a. It is concluded that aggregation decreases the reactivity of chlorophylls towards NaBH 4 in vitro, and may similarly decrease reactivity in naturally‐occurring chlorophyll‐protein complexes.