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PHOTODYNAMIC INACTIVATION OF RUBELLA VIRUS ENHANCES RECOMBINATION WITH A LATENT VIRUS OF A BABY HAMSTER KIDNEY CELL LINE BHK21*
Author(s) -
Yamamoto Nobuto,
Urade Masahiro
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04169.x
Subject(s) - rubella virus , virology , hamster , virus , rubella , baby hamster kidney cell , biology , cell culture , microbiology and biotechnology , vaccination , genetics , measles
Abstract— Rubella virus is very sensitive to photodynamic action. When tested with 1.2 times10 ‐5 M toluidine blue and 8 W fluorescent lamp at a fluence of 11 W/m 2 , inactivation kinetics showed a linear single hit curve with a k value of 1.48 min ‐1 . Photodynamic inactivation of rubella virus greatly enhanced recombination with a latent virus (R‐virus) of baby hamster kidney BHK21 cells. In contrast, no hybrids were detected in lysates of the cells infected with either UV‐treated or untreated rubella virus. Therefore, hybrid viruses were readily detected only in lysates of BHK21 cells infected with photodynamically treated rubella virus. Photodynamic damage of rubella virus genomes generated a new hybrid type (hybrid type 3) in addition to a previously described type 2 hybrid (formerly designated as HPV‐RV variant). Although both of these hybrid types carry the CF antigens of rubella virus, plaque forming ability of type 3 hybrid is neutralized neither by anti‐rubella serum nor by anti‐latent virus serum while type 2 hybrid is neutralized by anti‐latent virus serum.