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SINGLET MOLECULAR OXYGEN QUENCHING BY SATURATED AND UNSATURATED FATTY‐ACIDS AND BY CHOLESTEROL
Author(s) -
VeverBizet C.,
Dellinger M.,
Brault D.,
Rougee M.,
Bensasson R. V.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04165.x
Subject(s) - quenching (fluorescence) , singlet oxygen , chemistry , reaction rate constant , singlet state , double bond , benzene , triplet state , oxygen , photochemistry , unsaturated fatty acid , polyunsaturated fatty acid , analytical chemistry (journal) , fatty acid , molecule , chromatography , organic chemistry , fluorescence , kinetics , excited state , physics , quantum mechanics , nuclear physics
— The rate constants of molecular singlet oxygen quenching by saturated and unsaturated fatty‐acids and by cholesterol‐membrane critical components ‐ membrane critical components ‐ have been measured by time resolved detection of the 1270 nm phosphorescence of singlet molecular oxygen [O 2 ( 1 δ g )]. We have determined (i) an increment of 5.7 times 10 2 M ‐1 s ‐1 per ‐CH 2 ‐ in C 6 D 6 and CD 3 OD for saturated fatty acids between C 4 and C 20 , (ii) an increment of 3 times 10 4 M ‐1 s ‐1 per non‐conjugated cu‐double bond for C 18 unsaturated fatty acids, identical in C 6 D 6 and CD 3 OD, (iii) a lower quenching rate constant by a factor of 2.7 for the trans‐C 16 and trans‐C 18 as compared to the corresponding cis‐monounsaturated fatty acids, (iv) a rate constant of O 2 ( 1 δ g ) quenching by cholesterol of 5.7 times 10 4 M ‐l s ‐l in benzene. These rate constants are compared to those obtained for other membrane cellular components.