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INVESTIGATION OF DAMAGE TO FOREST BY EPR SPECTROSCOPY in vivo
Author(s) -
Stegmann H. B.,
Schuler P.,
Ruff H.J.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04149.x
Subject(s) - electron paramagnetic resonance , p700 , hyperfine structure , spectroscopy , thylakoid , spectral line , chemistry , chloroplast , photosystem i , analytical chemistry (journal) , nuclear magnetic resonance , photosystem ii , photosynthesis , physics , atomic physics , environmental chemistry , biochemistry , astronomy , gene
— Spruce needles collected from several trees of the Black Forest were investigaled by EPR spectroscopy. These needles show in the g = 2.00 region a signal II S (Tyr D + ) and a light‐induced signal I(P700 + ) and a Mn 2+ hyperfine structure which superimposes the other absorptions. Difference spectra, light minus dark, partly eliminate the manganese hyperfine structure, and P700 + can be observed. By comparison of these EPR signals with those of spinach chloroplast or thylakoid membranes described in the literature, significant deviations were observed, whereas several trees grown in the vicinity of Tubingen exhibit the well known D + and P700 + EPR spectra. After treatment of branches of these ‘normal’ trees with herbicides like Amitrol and Roundup or chemicals like toluene or trichlormethane the EPR signals obtained are comparable with those observed with needles of the Black Forest.