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RECOVERY OF VIRAL CAPACITY IN IRRADIATED EXPONENTIALLY GROWING CELLS
Author(s) -
Sayed Nadira I.,
Rupert Claud S.
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04141.x
Subject(s) - exponential growth , irradiation , microbiology and biotechnology , biophysics , biology , chemistry , virology , physics , nuclear physics , quantum mechanics
— Exponentially growing cells of the PtK‐2 line (ATCC No. CCL56, from the marsupial Potorous tridactylus) require protein and RNA synthesis in a limited period following UV‐radiation damage for optimal recovery as colony formers [Overberg et al. (1988) Mutat. Res. 194, 83–92]. Overall behavior suggests the operation of damage‐induced recovery processes. The capacity of confluent cell monolayers for infection with unirradiated herpes simplex virus 1 (HSV‐1) is sharply reduced by UV‐irradiation. We have followed capacity changes in exponentially growing cells after irradiation and varying amounts of photoreactivation by means of an infectious center assay. These changes closely parallel changes of colony formation. Spontaneous recovery of capacity in the dark occurs over approximately the same time period that the UV sensitivity of colony formation depends on macromolecular synthesis. The effect of photoreactivation is complementary rather than additive to this recovery, suggesting that the dark recovery in this period concerns pyrimidine dimers in cell DNA.