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LIGHT PERCEPTION BY PHYTOCHROME in vivo*
Author(s) -
Mancinelli Alberto L.,
Lim Jui
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04138.x
Subject(s) - phytochrome , in vivo , cucurbita pepo , etiolation , biophysics , biology , botany , biochemistry , red light , genetics , enzyme
— The rate of phytochrome photoconversion in cotyledons and hypocotyls of dark‐grown seedlings of Cucurbita pepo varies significantly depending on the characteristics of the light field and the position of the sample in the light field. The overall pattern of the effects of light reflectance conditions and angle of incidence of light on phytochrome photoconversion in vivo is predictable and consistent with the results of physiological studies; however, the extent of the effects is much more difficult to predict with a satisfactory degree of approximation. The differences between phytochrome photoconversion kinetics measured in vivo and those predicted from photochemical parameters of purified phytochrome are rather large and suggest that the use of predictions as estimates of the state of phytochrome in vivo may be subject to significant limitations.