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COMPARISON OF PHAGE T4 denV ENDONUCLEASE V AND M. Luteus UV‐DNA ENDONUCLEASE BY SEROLOGY AND DNA HYBRIDIZATION
Author(s) -
YAROSH DANIEL B.,
CECCOLI JOSEPH
Publication year - 1989
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1989.tb04077.x
Subject(s) - microbiology and biotechnology , endonuclease , dna , micrococcus luteus , restriction enzyme , biology , gene , bacteriophage , chemistry , biochemistry , escherichia coli
Antibodies were raised in rabbits against purified endonuclease V, the product of the bacteriophage T4 denV gene, which incises DNA at the site of UV‐induced pyrimidine dimers. These antibodies cross‐reacted with the purified UV‐DNA endonuclease from M. luteus in both enzyme‐linked immunosorbent assay and Western assays. However, no sequence similarity was detected between the denV gene and M. luteus DNA by hybridization. The two endonucleaes are remarkably similar in enzymatic activity, and their antigenic similarities have been preserved despite differences in their DNA sequences.