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RED CHEMILUMINESCENCE FROM RAM SEMINAL VESICLE MICROSOMES: PITFALLS IN THE USE OF SPECTRALLY RESOLVED RED CHEMILUMINESCENCE AS A TEST FOR SINGLET OXYGEN IN BIOLOGICAL SYSTEMS
Author(s) -
Kanofsky Jeffrey R.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb08851.x
Subject(s) - chemiluminescence , singlet oxygen , chemistry , oxygen , photochemistry , microsome , singlet state , analytical chemistry (journal) , chromatography , biochemistry , organic chemistry , in vitro , excited state , atomic physics , physics
— The assignment of the red chemiluminescence bands in the ram seminal microsome system to singlet oxygen as previously reported by Cadenas and Sies (1983) Hoppe‐Seyler's Z. Physiol. Chem. 364, 519‐528 was re‐evaluated. Measurements of 1268 nm emission demonstrated that ram seminal vesicle microsomes produced very small quantities of singlet oxygen (0.41 2 0.05 p.M) when they metabolized high concentrations of 15‐hydroperoxyarachidonic acid (1 mM). The red chemilumi‐ nescence, however, was not due to singlet oxygen, since it failed to increase in deuterium oxide and it was five orders of magnitude larger than the predicted emission from singlet oxygen produced in this system. Quantitative measurements of the time integral of the square root of the red chemiluminescence intensity may be a useful test to confirm the assignment of red emission to singlet oxygen in other biochemical systems.