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EFFECTS OF INTENSITY AND FLUENCE UPON DNA SINGLE‐STRAND BREAKS INDUCED BY EXCIMER LASER RADIATION
Author(s) -
Chilbert M. A.,
Peak M. J.,
Peak J. G.,
Pellin M. J.,
Gruen D. M.,
Williams G. A.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb08839.x
Subject(s) - fluence , excimer laser , laser , irradiation , pulse (music) , radiation , bacillus subtilis , materials science , excimer , intensity (physics) , dna , analytical chemistry (journal) , chemistry , optics , atomic physics , chromatography , biology , physics , bacteria , biochemistry , detector , nuclear physics , genetics
— A Xenon‐chloride excimer laser emitting energy at 308 nm was used to induce single‐strand breaks (SSBs, frank breaks plus alkali‐labile lesions as assayed by alkaline sucrose sedimentation techniques) in purified DNA from Bacillus subtilis . A fluence response study and a peak pulse intensity study were performed. At a pulse energy of 0.1 mJ/pulse, the radiation induced SSBs in a linear fashion (91 SSB/10 8 Da per MJ/m 2 ) to a maximum exprimental fluence of 1.28 MJ/m 2 . The pulse intensity study showed that there were no significant changes in DNA breakage (105 SSB/10 8 Da) between 2.93 times 10 9 and 5.86 times 10 11 W/m 2 (0.11 and 22.0 mJ/pulse) at a constant total fluence of 1.1 MJ/m 2 (27000 mJ dose). This study has verified and extended previous work by quantifying the yield of SSBs induced in DNA by this laser radiation.