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CHLORINATION OF ISOLATED CHLOROPHYLLS in vitro
Author(s) -
Senge Mathias,
Senger Horst
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02885.x
Subject(s) - thin layer chromatography , chlorophyll , chemistry , epimer , chlorophyll a , pigment , silica gel , chloride , nitrogen , chromatography , thin layer , atmosphere (unit) , layer (electronics) , organic chemistry , biochemistry , meteorology , physics
— It was shown that chlorophyll a and its epimer chlorophyll a' are chlorinated and hydroxyl‐ated during thin layer chromatography with silica gel plates. Hydroxylated chlorophyll a could not be chlorinated. Chlorinated and non chlorinated chlorophylls could be separated and determined by high performance liquid chromatography, because chlorophylls do not alter during this procedure. These findings support the assumption that Chi RC I is a preparation artifact. The extent of chlorination depends on chloride availability on the thin layer and on time. Chlorination and hydroyxlation do not take place under nitrogen atmosphere. The mechanism of chlorination during the thin layer chromatography procedure is discussed.