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IMPAIRED REPAIR OF UVC‐INDUCED DNA DAMAGE IN L5178Y‐R CELLS: DNA UNWINDING STUDIES WITH THE USE OF 1‐β‐D‐ARABINOFURANOSYL CYTOSINE
Author(s) -
Szumiel Rena,
Wlodek Danuta,
Johanson KarlJ.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02807.x
Subject(s) - dna , microbiology and biotechnology , thymidine , cytosine , dna damage , dna repair , chemistry , biophysics , biology , biochemistry
— L5178Y‐R (LY‐R) and L5178Y‐S (LY‐S) cells differ in sensitivity to UVC radiation (D 0 : 2.8 and 9.0 J/m 2 respectively, for cells exposed in Fischer's medium). We used these cells and a DNA unwinding technique in conjunction with 1‐β‐D‐arabinosyl cytosine to determine DNA strand breaks accumulating as a result of enzymatic incision during DNA repair. Following UVC exposure DNA strand break accumulation was observed in LY‐S cells but not in LY‐R cells. The repair defect in LY‐R cells is accompanied by a delayed recovery of [ 3 H]thymidine incorporation.