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BINDING, EXCITATION ENERGY TRANSFER AND PHOTOSENSITIZATION IN GRISEOFULVIN‐DNA MIXTURES
Author(s) -
Neely W. C.,
Siraj M. Y.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02804.x
Subject(s) - griseofulvin , chemistry , quenching (fluorescence) , fluorescence , titration , dna , in vivo , guanosine , energy transfer , photochemistry , biophysics , biochemistry , biology , medicine , physics , microbiology and biotechnology , pathology , quantum mechanics , molecular physics
— The weak and reversible binding of the antifungal drug, griseofulvin (GF), to calf thymus DNA has been demonstrated by difference spectroscopy and the quenching of the fluorescence of GF by DNA observed. The value of K n was determined to be 800 M ‐1 by fluorescence quenching titration. Adenosine and guanosine also exhibit difference spectra with GF and quench GF fluorescence indicating that they may be the site of both binding and energy transfer. The in vitro photosensitization of DNA by griseofulvin is shown to occur. It is proposed that the clinically observed in vivo photosensitizing action of griseofulvin may result from binding followed by excitation energy transfer and that this may also be important in the antifungal activity of the drug.