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MONOCLONAL ANTIBODY WITH SPECIFIC AFFINITY FOR PYRIMIDINE DIMERS IN RNA
Author(s) -
Phillips Roger G.,
Kalthoff Klaus
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02789.x
Subject(s) - pyrimidine , chemistry , uridine , dimer , pyrimidine dimer , nucleotide , rna , monoclonal antibody , antibody , microbiology and biotechnology , stereochemistry , biochemistry , biology , dna , immunology , organic chemistry , dna damage , gene
— A monoclonal antibody was prepared which meets three criteria for specific binding to pyrimidine dimers in RNA. (i) UV irradiation at wavelengths greater than 300 nm in the presence of a triplet state sensitizer, or at 270 nm without sensitizer, promotes antibody binding to RNA and polyribonucleotides, (ii) Antibody binding is reduced by exposure to UV radiation of short wavelength (240 nm) following sensitized irradiation (<300 nm). (iii) Antibody binding is dependent upon the presence of adjacent pyrimidine ribonucleotides. The antibody recognizes a single uridine dimer with one or more additional nucleotides at both ends. Affinity for a single uridine dimer with additional nucleotides at only the 3′ end is substantially weaker.