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PHOTOSENSITIZATION BY ANTITUMOR AGENTS–7. CORRELATION BETWEEN ANTHRACENEDIONE‐PHOTOSENSITIZED DNA DAMAGE, NADH OXIDATION AND OXYGEN CONSUMPTION FOLLOWING VISIBLE LIGHT ILLUMINATION
Author(s) -
Hartley John A.,
Reszka Krzysztof,
Lown J. William
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02781.x
Subject(s) - anthraquinones , dna , chemistry , oxygen , dna damage , phototoxicity , photochemistry , base pair , mitoxantrone , intercalation (chemistry) , biophysics , anthraquinone , biochemistry , biology , organic chemistry , genetics , in vitro , botany , chemotherapy
— The 1,4‐diamino‐substituted anthraquinones mitoxantrone and ametantrone do not photosensitize DNA damage following illumination with visible light. In contrast, both the 1,5‐ and 1,8‐bis[[(diethylamino)ethyl]amino]anthraquinones, AMI and AM2 respectively, sensitized DNA single‐strand break formation in closed‐circular plasmid DNA upon exposure to visible light. The presence of an electron donor such as NADH is required in the case of AMI, and enhances the effect with AM2. At increasing DNA base pair to drug ratios the rate of oxygen consumption decreased rapidly suggesting that the drug photosensitizing properties are lost upon binding or intercalation into DNA. A direct correlation between oxygen consumption, NADH oxidation and extent of DNA damage was established at different DNA base pair to drug ratios.