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PHOTOCHEMICAL PROPERTIES OF ERYTHROCYTE GHOSTS CONTAINING PORPHYRIN
Author(s) -
FIREY P. A.,
RODGERS M. A. J.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02756.x
Subject(s) - porphyrin , photochemistry , phosphorescence , singlet oxygen , chemistry , suspension (topology) , flash photolysis , luminescence , fluorescence , oxygen , triplet state , kinetics , materials science , reaction rate constant , molecule , optoelectronics , optics , organic chemistry , physics , mathematics , quantum mechanics , homotopy , pure mathematics
— Rabbit erythrocyte cell ghosts were prepared containing tetrasodium meso‐tetra (4‐sulfona‐to‐phenyl) porphine (12‐hydrate), a water soluble porphyrin. Laser flash kinetic spectroscopy of the suspension revealed a difference spectrum consistent with the porphyrin T, state. The time profiles of the signal decay were bi‐exponential, and oxygen quenched each of the two components differently. Triplet‐triplet extinction coefficients and quantum yields were lower for the ghost suspension than for porphyrin in solution. Fluorescence lifetime studies of the suspension also showed the presence of two components. Singlet oxygen luminescence, as measured by germanium diode detection, was observed, with a lifetime of 46 (jls and a 4>A of 0.09.