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124‐kDa PHYTOCHROME IN MODEL MEMBRANE SYSTEMS: ASSOCIATION STUDIES AND COVALENT BINDING TO PREFORMED LIPOSOMES
Author(s) -
Krieg Marianne,
Braslavsky Silvia E.,
Schaffner Kurt
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02731.x
Subject(s) - liposome , phytochrome , phosphatidylcholine , biophysics , chemistry , membrane , egg lecithin , lecithin , biochemistry , covalent bond , chromatography , biology , phospholipid , botany , organic chemistry , red light
— 124‐kDa Phytochrome from oat has been covalently bound to the surface of preformed unilamellar liposomes doped with functionalized lipids. The extent of phytochrome binding varied from 100% (to soybean lecithin and dioleoyl phosphatidylcholine liposomes) to (90 ± 10)% (to dimyristoyl phosphatidylcholine liposomes) and (50 ± 10)% to dipalmitoyl phosphatidylcholine liposomes). The photochromic properties of phytochrome were fully retained in the liposome‐bound systems. Attempts to bring about spontaneous incorporation and binding of the phytochrome to neutral and positively charged liposomes failed. These results were independent of liposome size, the presence of cholesterol, and whether phytochrome was added prior to or after the liposome formation.