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PHOTOREACTIVATION OF Halobacterium halobium: ACTION SPECTRUM AND ROLE OF PIGMENTATION
Author(s) -
Iwasa Tatsuo,
Tokutomi Satoru,
Tokunaga Fumio
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02725.x
Subject(s) - photolyase , action spectrum , pigment , carotenoid , biology , chromophore , cleavage (geology) , botany , photochemistry , chemistry , biochemistry , dna , dna repair , organic chemistry , paleontology , fracture (geology)
— An action spectrum for photoreactivation was measured with Halobacterium halobium R 1 m 1 to prove a role of carotenoid pigments in photoreactivation of the bacteria. The action spectrum obtained showed a main peak at 435 nm and a minor peak at about 325 nm. The action spectrum was similar to that of Streptomyces pigment (Eker et al. , 1981) suggesting that the chromophore of the photoreactivating enzyme in Halobacterium halobium is 8‐OH‐5‐deazaflavin. The minor peak may be due to photochemical cleavage of a pyrimidine6–4 hetero adduct. The result indicates that carotenoid pigments do not play a positive role in enhancing photoreactivation. This was confirmed also by comparing the efficiency of photoreactivation at 465 nm among three strains of Halobacterium halobium having different carotenoid pigments; R 1 m 1 . R 1 and W5002–1.