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PRODUCTION OF PHOTOREACTIVEABLE LESION IN THE YEAST S. cerevisiae BY IRRADIATION WITH 193 nm EXCIMER LASER LIGHT
Author(s) -
Winckler K.,
Golz B.,
Laskowski W.,
Bende T.
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02719.x
Subject(s) - pyrimidine dimer , photolyase , excimer laser , irradiation , laser , saccharomyces cerevisiae , strain (injury) , excimer , yeast , lesion , radiation , chemistry , optics , materials science , dna damage , dna , dna repair , biology , anatomy , biochemistry , surgery , medicine , physics , nuclear physics
— Far‐UV (193nm) excimer‐laser irradiation of an excision‐repair defective but photoreactivatiozn competent strain of Saccharomyces cerevisiae (XS774–6A) produces a significant amount of photoreactiveable DNA‐lesions. The irradiation was applied by a laser planned for micro‐surgery in ophthalmology using doses and dose rates even lower as needed during the surgical use. Irradiation experiments on solid agar plates show a sharp bordered area of growth‐inhibition around the impact of the laser‐beam up to a diameter of 2 cm even for a radiation‐repair competent wildtype strain. In this area photoreactivation of cells is detectable, proving the induction of pyrimidine‐dimers in cells surrounding the laser‐beam impact zone.