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PICOSECOND FLUORESCENCE SPECTROSCOPY ON INCORPORATION PROCESSES OF HEMATOPORPHYRIN DERIVATIVE INTO MALIGNANT TUMOR CELLS in vitro
Author(s) -
YAMASHITA MIKIO,
TOMONO TAKAHISA,
KOBAYASHI SHYUNSUKE,
TORIZUKA KENJI,
AIZAWA KATSUO,
SATO TAKUZO
Publication year - 1988
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1988.tb02712.x
Subject(s) - hematoporphyrin , fluorescence , picosecond , streak camera , chemistry , fluorescence spectroscopy , in vitro , spectroscopy , incubation , time resolved spectroscopy , photochemistry , biophysics , analytical chemistry (journal) , optics , biology , photodynamic therapy , biochemistry , chromatography , physics , organic chemistry , quantum mechanics , laser
— By using a highly sensitive streak‐camera technique, we investigate incorporation processes of HpD into malignant tumor m‐KSA cells in vitro. The picosecond decays of the total fluorescence spectra, the wavelength‐resolved fluorescence decays and the time‐resolved fluorescence spectra from HpD in the cells are measured as a function of the incubation time. The results show that the aggregate component of HpD which has a fast fluorescence lifetime of 100 ps and a red‐shifted band of ∼ 660 nm selectively accumulates more and more in the cells with the increase of the incubation time.