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PURIFICATION OF PHYTOCHROME FROM RYE BY FAST PROTEIN LIQUID CHROMATOGRAPHY
Author(s) -
Ernst D.,
Vojacek R.,
Oesterhelt D.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb07896.x
Subject(s) - fast protein liquid chromatography , phytochrome , hydroxylapatite , chromatography , fractionation , chemistry , high performance liquid chromatography , biochemistry , biology , botany , red light , enzyme
. A rapid procedure for the purification of phytochrome from rye by fast protein liquid chromatography (FPLC) is described. A pre‐purification with Q‐Sepharose fast flow and hydroxylapatite fast flow is necessary in order not to exceed the total capacity of the Mono‐Q HR 5/5 column. The whole fractionation is finished within 24 h and yields an intact 124‐kDa phytochrome of high purity.