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THE USE OF SPECIFIC RADIOIMMUNOASSAYS TO DETERMINE ACTION SPECTRA FOR THE PHOTOLYSIS OF (6‐4) PHOTOPRODUCTS
Author(s) -
Mitchell David L.,
Rosenstein Barry S.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb07882.x
Subject(s) - photodissociation , radioimmunoassay , chemistry , photochemistry , action spectrum , absorption spectroscopy , spectral line , optics , biochemistry , physics , astronomy
. A radioimmunoassay (RIA) was developed which specifically detects a photoproduct produced by the near‐UV photolysis of pyrimidine(6‐4)pyrimidone photoproducts. This assay was used in conjunction with a previously characterized RIA which specifically detects (6‐4) photoproducts to determine the relative efficiency of wavelengths between 265 and 435 nm for photolysing these lesions. The rate of loss of antibody‐binding sites associated with (6‐4) photoproducts correlates with the production of those associated with its photolysis product. Action spectra for both the loss of (6‐4) photoproducts and the induction of the photolysis product parallel the absorption spectrum of the (6‐4) photoproduct.