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TEMPERATURE DEPENDENCE OF PHOSPHORESCENCE PARAMETERS OF PHYLOGENETICALLY DISTANT APOMYOGLOBINS
Author(s) -
Bismuto Ettore,
Strambini Giovanni B.,
IRACE GAETANO
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb07875.x
Subject(s) - phosphorescence , sperm whale , whale , chemistry , flexibility (engineering) , crystallography , photochemistry , myoglobin , physics , biology , optics , biochemistry , fluorescence , ecology , statistics , mathematics
. The triplet state lifetime and the position of the O—O vibrational band of the tryptophanyl phosphorescence spectrum of two philogenetically distant apomyoglobins, i.e. tuna and sperm whale apomyoglobin, have been studied at different temperatures. The temperature dependence of the two phosphorescence parameters revealed that the tryptophanyl microenvironment possesses different dynamic characteristics in the two examined proteins. In particular, the tryptophanyl side chain of tuna apomyoglobin was found embedded into a less rigid microenvironmental cluster which possesses a higher degree of internal flexibility than that of sperm whale apomyoglobin.