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DETECTION OF CHARGE‐PERTURBATION OF TRYPTOPHAN RESIDUES BY FOURTH‐DERIVATIVE SPECTROPHOTOMETRY
Author(s) -
Sabés Manuel,
Padrós Esteve
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb07394.x
Subject(s) - tryptophan , spectrophotometry , indole test , chemistry , chromophore , derivative (finance) , photochemistry , stereochemistry , chromatography , biochemistry , amino acid , financial economics , economics
— Fourth‐derivative spectrophotometry has been applied to the analysis of the 1 L a O‐O band of the indole chromophore. Using several tryptophan models, it is shown that when this band is the most red‐shifted, it produces a minimum at about 300 nm in the fourth‐derivative spectrum. In proteins this red shift and the 300‐nm minimum results from a charge‐perturbation effect acting through space on the indole group of tryptophan residues. It is concluded that fourth‐derivative spectrophotometry can be used for monitoring the effect of charges on tryptophan residues