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PRINCIPLES AND APPLICATIONS OF FLUORESCENCE TECHNIQUES IN BIOPHYSICAL CHEMISTRY *
Author(s) -
Geacintov Nicholas E.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb05417.x
Subject(s) - fluorophore , fluorescence , chemistry , biopolymer , fluorescence in the life sciences , quenching (fluorescence) , macromolecule , ligand (biochemistry) , nucleic acid , fluorescence spectroscopy , biomolecule , photochemistry , organic chemistry , polymer , biochemistry , optics , physics , receptor
— Fluorescence is widely used as a probe of the properties of ligand‐biopolymer complexes or adducts, proteins, nucleic acids and membranes. The principles and techniques utilized in characterizing the basic fluorescence properties of a system of fluorophore‐biopolymer complexes are outlined. The different approaches utilized include a correlation of fluorescence decay profiles obtained by time‐correlated single photon counting techniques with (1)steady‐state fluorescence excitation and emission spectra, (2)relative fluorescence quantum yields, (3)fluorophore‐accessibilities investigated by quenching techniques, and (4)ligand‐macromolecule binding equilibria.