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PHOTOREACTIVATION OF ULTRAVIOLET RADIATION DAMAGE IN DARK REPAIR DEFICIENT PHR MUTANTS OF Escherichia coli K‐12
Author(s) -
Smith A. W.,
Davies D. J. G.,
Moss S. H.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb05372.x
Subject(s) - photolyase , mutant , locus (genetics) , biology , genetics , gene , escherichia coli , ultraviolet radiation , microbiology and biotechnology , dna repair , chemistry , radiochemistry
There is uncertainty in the literature concerning the genetic control of photoreactivation in E. coli. Two genetic loci, phrA and phrB have been proposed, and two photolyase molecules have been isolated, but in vivo evidence for the activity of the former is controversial. We have studied photoreactivation after 254 nm UV in a dark‐repair‐deficient phrB mutant and in a strain deleted at the proposed phrA locus. We show apparent photoenzymatic repair in the phrB mutant, which is abolished when the mutation is transduced into the proposed phrA deletion mutant. We conclude that there is a gene in the region of the proposed phrA locus which affects photoenzymatic repair.