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In vivo 31 P‐NMR SPECTROSCOPY OF MAMMARY CARCINOMA SUBJECTED TO SUB CURATIVE PHOTODYNAMIC THERAPY
Author(s) -
Chopp M.,
Farmer H.,
Hetzel F.,
Schaap A. P.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04853.x
Subject(s) - in vivo , mammary carcinoma , photodynamic therapy , nuclear magnetic resonance spectroscopy , spectroscopy , chemistry , carcinoma , nuclear magnetic resonance , medicine , pathology , biology , stereochemistry , physics , microbiology and biotechnology , organic chemistry , quantum mechanics
In vivo31 P‐NMR spectroscopy was applied to the investigation of the time course of metabolic response of MCA mammary carcinoma in the C,H mouse subjected to a subcurative treatment with photodynamic therapy. Eleven animals were injected with 12.5 mg kg _1 Photofrin II and after 24 h were photoactivated with 25 J cm −2 of light between 630 ± 5 nm. In vivo NMR spectroscopy was performed 1‐4, 24 and 72 h after treatment. The ratio of (J‐ATP intensity to inorganic phosphate (Pi) intensity changed over the time course of the study. At the 1‐4 and 24 h time points the ratio of fi‐ATP to Pi decreased from control values. At 72 h there was a significant increase in the ratio above the post treatment values to levels equal to or exceeding that of control pretreatment ratios.