Premium
QUANTITATIVE in vivo MEASUREMENT OF THE FLUORESCENT COMPONENTS OF PHOTOFRIN II
Author(s) -
Schneckenburger Herbert,
Feyh Jens,
Gotz Alwin,
Frenz Martin,
Brendel Walter
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04845.x
Subject(s) - hematoporphyrin , fluorescence , in vivo , chemistry , porphyrin , nanosecond , protoporphyrin ix , picosecond , biophysics , photodynamic therapy , monomer , photosensitizer , derivative (finance) , photochemistry , biology , optics , organic chemistry , laser , physics , microbiology and biotechnology , polymer , financial economics , economics
Photofrin II which contains the most efficient components of hematoporphyrin derivative with regard to photodynamic therapy of cancer, was measured fluorometrically in tumor and tumor‐free tissues in vivo over a period up to 8 days. Using time‐resolving (nanosecond and picosecond) microscopic techniques, the fluorescence of different components was quantitated and attributed to monomelic, dimeric, and possibly aggregated porphyrin species. The long‐lasting retention of the porphyrins in live tissues was in contrast to the rapid removal from cultured cells. This might be due to monomerization or dimerization of non‐fluorescent aggregates. Tumor‐selective accumulation was found to be similar for two different (probably monomeric and dimeric) components. This indicates that the integral fluorescence of these components may also correlate with the distribution of the main photosensitizing species.