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PHOTOSENSITIZING PROPERTIES OF BACTERIOCHLOROPHYLLIN a and BACTERIOCHLORIN a, TWO DERIVATIVES OF BACTERIOCHLOROPHYLL a
Author(s) -
Beems Eva M.,
Dubbelman Tom M. A. R.,
Lugtenburg Johan,
Van Best Jaap A.,
Smeets Monique F. M. A.,
Boegheim J. Pierre J.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04825.x
Subject(s) - chemistry , photosensitizer , bacteriochlorophyll , dithiothreitol , hematoporphyrin , porphyrin , photochemistry , guanosine , photodynamic therapy , absorption (acoustics) , tryptophan , singlet oxygen , histidine , biochemistry , oxygen , organic chemistry , photosynthesis , physics , acoustics , enzyme , amino acid
The photosensitizing properties of two water soluble derivatives of bacteriochlorophyll a , bacteriochlorophyllin a and bacteriochlorin a (lacking the central Mg‐ion) were investigated and compared to those of hematoporphyrin derivatives. At physiological pH the oxygen consumption rate of histidine, tryptophan, dithiothreitol and guanosine upon illumination was 3 to 4 times higher when bacteriochlorin a was used as photosensitizer than when hematoporphyrin derivatives were used. Especially bacteriochlorin a proved to be an effective sensitizer for the killing of L929 cells. Because bacteriochlorin a has an absorption maximum at 765 nm in phosphate buffered saline (allowing a light penetration in tissue about ten times larger than at 630 nm) and a high molar absorption coefficient (32 000 M cm −1 ) it has promising possibilities for the application in photodynamic therapy.