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ESTIMATION OF AN INDEX OF HYDROPHOBICITY OF DNA INTERIOR USING 5‐METHOXYPSORALEN AS A FLUORESCENT PROBE
Author(s) -
Sasaki Masako,
Nakasato Isao,
Sugiura Hiroyuki,
Fujita Hitoshi,
Sakata Toshibumi
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04810.x
Subject(s) - psoralen , fluorescence , dna , chemistry , aqueous solution , molecule , methanol , base pair , biophysics , organic chemistry , biochemistry , biology , optics , physics
The index of hydrophobicity of DNA interior was estimated by measuring fluorescence spectra of psoralen derivatives associated with DNA. The environment around 5‐MOP associated with DNA was as hydrophobic (D k = 34) as methanol, suggesting that the molecules reside at the space between the base‐pairs in B‐form DNA. This is also true for 8‐MOP. Thus, planar and aromatic molecules of 5‐ and 8‐MOP are more stable in the interior of DNA than in aqueous medium due to hydrophobic affinity.