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UV PHOTOBIOLOGY OF THE NEMATODE Caenorhabditis elegans: ACTION SPECTRA, ABSENCE OF PHOTOREACTIVATION and EFFECTS OF CAFFEINE
Author(s) -
Keller Cindy I.,
Calkins John,
Hartman Phillip S.,
Rupert Claud S.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04799.x
Subject(s) - photolyase , photobiology , action spectrum , caenorhabditis elegans , caffeine , irradiation , biology , escherichia coli , biophysics , chemistry , biochemistry , botany , dna repair , dna , physics , endocrinology , gene , nuclear physics
Action spectra for UV inactivation of reproduction in Caenorhabditis elegans have been obtained for strains N2 (wild‐type) and rad‐3 (radiation‐sensitive). Use of a dye laser radiation source, providing high intensity in a narrow wavelength band, has permitted more detail (14 wavelengths between 260 and 320 nm) than available in the spectra for other multicellular organisms. Overall sensitivity of N2 is similar to that of wild‐type Escherichia coli; that of rad‐3 is 30‐fold higher between 265 and 310 nm; relative sensitivity decreases above 310 nm but also seems to increase for irradiation below 265 nm. Tests for photoreactivation and for modification of survival by post‐irradiation treatment with caffeine were negative.