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STATIC and TIME‐RESOLVED FLUORESCENCE OF AN AMPHIPHILIC FLAVIN IN AEROSOL OT REVERSED MICELLES
Author(s) -
Visser Antonie J. W. G.,
Vos Kees,
Santema Jillert S.,
Bouwstra Jan,
Hoek Arie VAN
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04795.x
Subject(s) - flavin group , fluorescence , chemistry , micelle , pulmonary surfactant , fluorescence anisotropy , analytical chemistry (journal) , heptane , photochemistry , chromatography , aqueous solution , optics , organic chemistry , biochemistry , physics , enzyme
Spectral and time‐resolved fluorescence studies have been carried out on N(3)‐undecyllumi‐flavin dispersed in reversed micelles composed of the surfactant sodium di(2‐ethylhexyl) sulfosuccinate (Aerosol OT, AOT), various amounts of water and n ‐heptane as continuous phase. The fluorescence spectral properties (spectral distribution, quantum efficiencies and lifetimes) as function of the water to AOT molar ratio suggest that the flavin occupies a position within the surfactant boundary layer in close contact with water. The fluorescence anisotropy exhibits biexponential decay with a short (0.3 ns) and a longer (1.6–2.4 ns) correlation time. The contribution of the short component increases with the growth of the droplet providing evidence for enhanced flexibility of the flavin in the interfacial layer.