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IMPROVED QUANTITATION OF DNA‐PROTEIN CROSSLINKING CAUSED BY 405‐nm MONOCHROMATIC NEAR‐UV RADIATION IN HUMAN CELLS
Author(s) -
Peak Jennifer G.,
Peak Meyrick J.,
Blazek Ed Robert
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04774.x
Subject(s) - monochromatic color , fluence , dna , yield (engineering) , radiation , chemistry , ultraviolet , range (aeronautics) , ultraviolet radiation , analytical chemistry (journal) , biophysics , materials science , chromatography , optics , biochemistry , radiochemistry , biology , optoelectronics , organic chemistry , physics , ion , metallurgy , composite material
An improved alkaline elution analysis has been used to estimate the yield of DNA‐protein crosslinks induced in human P3 cells by monochromatic 405‐nm near‐ultraviolet radiation. Crosslinks are induced linearly over the fluence range studied, at a rate of 1.22 crosslinks per 10 10 daltons per MJ m ‐2 ± 0.066 (one standard error of the slope, N = 37). This rate is equivalent to 1360 crosslinks per cell genome per lethal event.