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AN ACTION SPECTRUM FOR LETHAL PHOTOSENSITIZATION OF Candida albicans BY 8‐MOP AFTER LOW‐DOSE BROAD‐BAND UV‐A IRRADIATION; AN ACTION SPECTRUM FOR 8‐MOP 4′,5′‐ MONOADDUCTS?
Author(s) -
Baydoun Safa A.,
Young Antony R.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04772.x
Subject(s) - action spectrum , candida albicans , irradiation , broad spectrum , lethal dose , chemistry , photochemistry , broad band , methoxsalen , visible spectrum , biology , microbiology and biotechnology , materials science , optoelectronics , physics , optics , combinatorial chemistry , organic chemistry , psoriasis , nuclear physics , immunology
An action spectrum was obtained for lethal photosensitization of Candida albicans that had been pre‐irradiated with a sub‐lethal low dose of broad‐band UV‐A after incubation with 8‐methoxypsoralen and then washed to remove any non‐photobound 8‐methoxypsoralen. The resultant spectrum with a peak in the 320‐335 nm region was similar to that obtained by a conventional method (single irradiation only) and also to the absorption spectrum of the 4′,5′‐monoadduct. These data support the hypothesis that the chromophore for lethal photosensitization by 8‐methoxypsoralen is the 8‐methoxypsoralen/DNA 4′,5′‐monoadduct rather than 8‐methoxypsoralen itself.