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MENADIONE SENSITIZED PHOTOOXIDATION OF NUCLEIC ACID and PROTEIN CONSTITUENTS. AN ESR and SPIN‐TRAPPING STUDY
Author(s) -
Krishna C. Murali,
Decarroz C.,
Wagner J. R.,
Cadet J.,
Riesz P.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04754.x
Subject(s) - menadione , chemistry , thymine , spin trapping , uracil , cytosine , adduct , photochemistry , nucleic acid , radical , triplet state , electron paramagnetic resonance , dna , biochemistry , organic chemistry , molecule , enzyme , physics , nuclear magnetic resonance
The menadione photosensitized reactions of nucleic acid and protein constituents were studied by ESR and spin trapping. Thymine, thymidine, cytosine, 2′‐deoxycytidine,5′‐dCMP, uracil and several N‐acetyl amino acids and dipeptides were investigated. Photolysis at 335 nm was carried out in air‐saturated or Ar saturated DMSO : H 2 O (1 : 1, vol/vol) containing 10 3 M menadione and 10 ‐2 M 2‐methyl‐2‐nitrosopropane as the spin trap. The observed spin adducts were explained in terms of electron transfer from the substrate to the excited triplet state of menadione to form the radical cation of the substrate and the anion radical of menadione which was also detected by ESR.