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ESR DETECTION OF ENDOGENOUS ASCORBATE FREE RADICAL IN MOUSE SKIN: ENHANCEMENT OF RADICAL PRODUCTION DURING UV IRRADIATION FOLLOWING TOPICAL APPLICATION OF CHLORPROMAZINE
Author(s) -
Buettner Garry R.,
Motten Ann G.,
Hall Robert D.,
Chignell Colin F.
Publication year - 1987
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1987.tb04751.x
Subject(s) - chlorpromazine , chemistry , hairless , radical , endogeny , electron paramagnetic resonance , irradiation , photochemistry , oxidative stress , biophysics , pharmacology , biochemistry , medicine , biology , nuclear magnetic resonance , physics , nuclear physics
Using electron spin resonance spectroscopy, we observed that UV radiation (330 nm) increased the endogenous ascorbate free radical concentration in hairless mouse (HRS/J) skin. When the skin was topically treated with a chlorpromazine solution prior to illumination, UV irradiation caused the ascorbate free radical concentration to increase even more. This observation suggests that there is an increased UV‐induced oxidative stress in the presence of chlorpromazine, probably caused by the production of free radicals from chlorpromazine.