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REPAIR OF CYCLOBUTANE DIMERS AND (6–4) PHOTOPRODUCTS IN ICR 2A FROG CELLS
Author(s) -
Mitchell David L.,
Clarkson Judith M.,
Chao Chuck C.K.,
ROSENSTEIN Barry S.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb09539.x
Subject(s) - cyclobutane , pyrimidine dimer , chemistry , irradiation , photochemistry , photolyase , dna , dna repair , biophysics , stereochemistry , biology , biochemistry , ring (chemistry) , organic chemistry , physics , nuclear physics
— The removal of cyclobutane dimers and Pyr(6–4)Pyo photoproducts from the DNA of UV‐irradiated ICR 2A frog cells was determined by radioimmunoassay. In the absence of photoreactivat‐ing light, 15% of the cyclobutane dimers and 60% of the (6–4) photoproducts were removed 24 h post‐irradiation with 10 J m −2 , Exposure to 30 kJ m −2 photoreactivating light resulted in removal of 80% of the cyclobutane dimers and an enhanced rate of repair of (6–4) photoproducts, resulting in a loss of 50% of these lesions in 3 h. The preferential removal of (6–4) photoproducts by excision repair resembles previously published data for mammalian cells.