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PHOTODYNAMIC EFFECTS INDUCED BY THE LUCIFERIN/LUCIFERASE SYSTEM
Author(s) -
Steveninck J.,
Boegheim J. P. J.,
Dubbelman T. M. A. R.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb09516.x
Subject(s) - luciferin , bioluminescence , luciferase , chemistry , luciferases , photochemistry , sodium azide , biophysics , dithiothreitol , light emission , biochemistry , enzyme , biology , optoelectronics , materials science , transfection , gene
— Luciferin from Photinur pyralis is an effective sensitizer, when excited with UV light in the range of 310–390 nm. With histidine or dithiothreitol as substrate, a type II photooxidation occurs, as judged from the inhibitory effect of sodium azide. During the ATP‐driven luciferin‐luciferase reaction, the resulting bioluminescence does not induce photodynamic reactions, as there is no overlap between the bioluminescence spectrum and the excitation spectrum of luciferin. However, in the presence of a second sensitizer, excitable by the bioluminescent light, photodynamic reactions can take place in the absence of exogenous light. As a consequence several photosensitizers can thus provoke photodynamic inactivation of luciferase.